Spanish Football Federation regional leaders have called on president Luis Rubiales to immediately resign after he kissed player Jenni Hermoso.
Rubiales, 46, has been widely criticised after the incident following Spain’s Women’s World Cup final win.
Hermoso, 33, said the kiss on her lips was not consensual.
Earlier, Spanish prosecutors opened a preliminary investigation into whether the incident amounts to a crime of sexual assault.
In other developments on Monday, Rubiales’ mother Angeles Bejar locked herself in a Spanish church and went on a hunger strike in protest of the treatment of her son. In the evening, hundreds of people gathered in the centre of Madrid in protests called by feminist groups in support of Hermoso and against Rubiales.
Rubiales was suspended on Saturday by world football’s governing body Fifa, a day after he had insisted he would not resign and claimed the kiss was “mutual and consensual”.
“After recent events and the unacceptable behaviour that has seriously damaged the image of Spanish football, the presidents request that Luis Rubiales resign immediately as president of the RFEF,” the federation (RFEF) said in a statement on Monday, after an extraordinary meeting of its regional presidents.